Engineering Anastomosis between Biological Perfused Vessel Networks and Endothelial Cell-lined Microfluidic Channels
نویسندگان
چکیده
This paper reports, for the first time, a microfluidic system that can reproduce the vascular anastomosis between a perfused vessel network in a 3D tissue chamber and an endothelial cell (EC)-lined microfluidic channel. By using a decoupling design of the microfluidic device, the physiological microenvironment including both the interstitial flow for vasculogenesis and shear stress for EC lining can be well controlled. To facilitate the anastomosis process, the basal-to-apical transendothelial flow as well as the vascular endothelial growth factor (VEGF) gradient are involved in promoting the sprouting angiogenesis from the EC monolayer into the fibrin gel.
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